Hematology date: Maxwell Wintrobe Raynaud

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Re-heated to 37 0 C below the melting collapsing and serum cryoglobulin was said and cryoglobulinemia Ig'lere of leaving the three types (I, II, III) have described the Atlas of Hematology (see Cryoglobulinaemia). Today's topic: How to cryoglobulin laboratory should be searched?
Serum cryoglobulins are searched. Syringes and tubes must be used in handling the blood sample do preheated. For example, the capture, transport, water bath temperature during the clot, and centrifugation at 37 any time fall below. Separated serum 4 0 'must be kept until the seventh day. Monoclonal (Type I) cryoglobulins every few hours immediately collapses. However, mixed (mixed) cryoglobulins (Type II and Type III), especially for the days when the collapse of Type III may be required. mg fe 7si8o22 oh 2
During the study of laboratory errors, and will be more so, for example, and leads to false-negative results. The most common sources of error, for example during transport and storage of heat at 37 to reach the cold precipitate as a result (kriyopresipitet) re-melting.
Cryoglobulin concentration, total protein amount and the value of the cold precipitate kriyokrit indirectly calculated. In type I usually 5 g / l in. In type II and III is much lower. Kriyokrit mg fe 7si8o22 oh 2 'cold precipitate in order to measure mg fe 7si8o22 oh 2 the hematocrit tube and quickly mg fe 7si8o22 oh 2 translated into a macro or micro.
Ig precipitation remelted kriyoglobülinin mg fe 7si8o22 oh 2 also made Immunofixation type (monoclonal or mixed) can be determined. Type I cryoglobulinemia serum monoclonal Ig cold residue was re-dissolved mg fe 7si8o22 oh 2 with immünofiksasyonu immünofiksasyonu identical results.
The above figure of a patient with multiple myeloma (λ myeloma IgG) in the patient's serum 37 0 (A), the refrigerator (4 0) to stand for 24 hours in serum (B), and the deposited cold precipitate after centrifugation of the tube light (C) is observed.
Hematology date: Maxwell Wintrobe Raynaud's trying to Johns Hopkins Hospital in consultation with the patient's complaints, a woman who called. Wintrobe'un look at the color of the blood count, hematocrit, plasma tübündeki infallible rule (see Complete Blood Count). The patient's plasma is very clear. Then refrigerate to examine the blood sample tube on the leaves and surrounded hematocrit. Again when the ice cabinet plasma tube is filled with a substance that acts intensive blurred. Eagerly putting his shirt pocket where the white tube service runs to the patient's doctor. Service chief has taken off in front of the tube out of his pocket turbidity disappeared. Wintrobe in 1933 with the diagnosis of myeloma protein in this wonderfull releases (MM Wintrobe. Hematology, the Blossoming of a Science, pp. 93, Lea & Febiger, 1985). This is the first case of cryoglobulinemia
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